Journal: Cancer Immunology, Immunotherapy : CII

Article Title: DNA hypo-methylation and expression of GBP4 induces T cell exhaustion in pancreatic cancer

doi: 10.1007/s00262-024-03786-3

Figure Lengend Snippet: GBP4 showed hypo-methylated status in PDAC. A Chromosomal distribution of the CpG sites in the regulatory regions of GBP4, which was visualized by MethPrime, and the primers for bisulfite sequencing. B Representative methylation profiles of CpG sites in the regulatory regions of GBP4 from adjacent normal tissues and PDAC tissues detected using bisulfite genomic sequencing. Open circles indicate unmethylated CpG sites and closed circles indicate methylated CpG sites. C Representative methylation profiles of CpG sites in the regulatory regions of GBP4 from normal pancreatic ductal epithelial cells (HPDE6-C7) and PDAC cells (Capan-1, CFPAC-1, AsPC-1, and Mia Paca-2) detected using bisulfite genomic sequencing. D Differentially methylated CpG sites of GBP4 between normal pancreatic ductal epithelial cells and PDAC cells. The data are shown as mean ± SD

Article Snippet: The human pancreatic cancer cell lines (Capan-1, Mia PaCa-1, CFPAC-1, AsPC-1) and the immortalized normal pancreatic ductal epithelial cells (HPDE6-C7) were obtained from ATCC.

Techniques: Methylation, Methylation Sequencing, Genomic Sequencing

Journal: bioRxiv

Article Title: Harnessing the adaptive potential of mechanoresponsive proteins to overwhelm pancreatic cancer dissemination and invasion

doi: 10.1101/190553

Figure Lengend Snippet: (A) Representative images across HPDE, Panc10.05, and AsPC-1 cell lines of the maximum accumulation of GFP alone, GFP-labeled myosin IIs and α-actinin 4, and mCherry-α-actinin 1, show peak intensity after applied stress in MPA mechanoresponse experiments. Scale bar = 7 μm. (B) Quantification of mechanoresponsiveness normalized as a ratio of fluorescence intensity at the tip (I p ) to the intensity at the opposite cortex (I o ). Medians plotted; *p<0.05, **p<0.005, ***p<0.0005.

Article Snippet: Human pancreatic ductal epithelial cells (HPDE) were obtained from Dr. Ming-Sound Tsao (University of Toronto, Ontario Canada) and human primary tumor-derived cells (Panc10.05) and human metastatically-derived cells (AsPC-1) were purchased from ATCC.

Techniques: Labeling, Fluorescence

Journal: bioRxiv

Article Title: Harnessing the adaptive potential of mechanoresponsive proteins to overwhelm pancreatic cancer dissemination and invasion

doi: 10.1101/190553

Figure Lengend Snippet: (A) Expression of myosin IIA (NMIIA), myosin IIB (NMIIB), myosin IIC (NMIIC), and actin in HPDE (normal pancreatic ductal epithelium), Panc4.03 (stage II primary tumor), Panc10.05 (stage II primary tumor), and AsPC-1 (stage IV ascites metastasis) cells, compared with HeLa lysate for the purposes of quantification. NMIIA and NMIIC increase in expression, while NMIIB decreases in expression in cancer cell lines. (B) Quantification of the cellular concentration of each myosin II paralog. (C) Expression of alpha-actinin 4 (ACTN4) and filamin B (FLNB) increase, while expression of alpha-actinin 1 (ACTN1) and filamin A (FLNA) do not change in cancer cell lines. (D) Quantification of western blots, examples shown in (C) , where numbers on the bars indicate n-values. *p<0.05, **p<0.005.

Article Snippet: Human pancreatic ductal epithelial cells (HPDE) were obtained from Dr. Ming-Sound Tsao (University of Toronto, Ontario Canada) and human primary tumor-derived cells (Panc10.05) and human metastatically-derived cells (AsPC-1) were purchased from ATCC.

Techniques: Expressing, Concentration Assay, Western Blot

Journal: Discover oncology

Article Title: Cancer-associated fibroblast-derived COL17A1 promotes gemcitabine resistance and tumorigenesis in pancreatic cancer cells by interacting with ACTN4.

doi: 10.1007/s12672-025-01825-8

Figure Lengend Snippet: Fig. 2 CAFs increases COL17A1 expression in GEM-resistant PC cells. A GSE192907 dataset showed that COL17A1 was highly expressed in resistant CAFs. B Western blotting analysis for COL17A1 expression in PANC-1/GR and BxPC-3/GR cells incubated with NF-CM or CAF-CM. C–E TCGA, CPTAC and GEPIA databases showed that COL17A1 expression was higher in PAAD tissues compared with the normal samples. F Kaplan–Meier Plotter predicted that high COL17A1 expression was associated with shorter survival times in PAAD patients. G qRT-PCR anal- ysis for COL17A1 expression in GEM-resistant or -sensitive PC tissues. H Western blotting analysis for COL17A1 expression in normal HPDE, parental PANC-1 and BxPC-3 cells as well as PANC-1/GR and BxPC-3/GR cells. *P < 0.05

Article Snippet: :(0123456789) Human pancreatic duct epithelial (HPDE) cell line (Cat#CL-0921, Procell) was cultured in keratinocyte serum-free medium containing epidermal growth factor, bovine pituitary extract (Cat#:17005042, Invitrogen, Carlsbad, CA, USA) and 1% penicillin/streptomycin (Cat#PB180120, Procell) at 37 °C with 5% CO2.

Techniques: Expressing, Western Blot, Incubation, Quantitative RT-PCR